Cleavage of the urokinase receptor (uPAR) on oral cancer cells: regulation by transforming growth factor – β1 (TGF-β1) and potential effects on migration and invasion. Magnussen SN, Hadler-Olsen E, Costea DE, Berg E, Jacobsen CC, Mortensen B, Salo T, Martinez-Zubiaurre I, Winberg JO, Uhlin-Hansen L, Svineng G., BMC Cancer. 2017 May 19;17(1):350. doi: 10.1186/s12885-017-3349-7.

Integrin α11 is overexpressed by tumour stroma of head and neck squamous cell carcinoma and correlates positively with alpha smooth muscle actin expression. Parajuli H, Teh MT, Abrahamsen S, Christoffersen I, Neppelberg E, Lybak S, Osman T, Johannessen AC, Gullberg D, Skarstein K, Costea DE., J Oral Pathol Med. 2017 Apr;46(4):267-275. doi: 10.1111/jop.12493.

Nano-TiO2 penetration of oral mucosa: in vitro analysis using 3D organotypic human buccal mucosa models. Konstantinova V, Ibrahim M, Lie SA, Birkeland ES, Neppelberg E, Marthinussen MC, Costea DE, Cimpan MR, J Oral Pathol Med. 2017 Mar;46(3):214-222. doi: 10.1111/jop.12469.


Nanodiamond modified copolymer scaffolds affects tumour progression of early neoplastic oral keratinocytes. Suliman S, Mustafa K, Krueger A, Steinmüller-Nethl D, Finne-Wistrand A, Osdal T, Hamza AO, Sun Y, Parajuli H, Waag T, Nickel J, Johannessen AC, McCormack E, Costea DE. Biomaterials. 2016 Jul;95:11-21. doi: 10.1016/j.biomaterials.2016.04.002.

Establishment of a bioluminescence model for microenvironmentally induced oral carcinogenesis with implications for screening bioengineered scaffolds. Suliman S, Parajuli H, Sun Y, Johannessen AC, Finne-Wistrand A, McCormack E, Mustafa K, Costea DE. Head Neck. 2016 Apr;38 Suppl 1:E1177-87. doi: 10.1002/hed.24187.

Gene Expression and Proteome Analysis as Sources of Biomarkers in Basal Cell Carcinoma. Lupu M, Caruntu C, Ghita MA, Voiculescu V, Voiculescu S, Rosca AE, Caruntu A, Moraru L, Popa IM, Calenic B, Greabu M, Costea DE. Dis Markers. 2016;2016:9831237. doi: 10.1155/2016/9831237. Review.

Establishment of 3D Co-Culture Models from Different Stages of Human Tongue Tumorigenesis: Utility in Understanding Neoplastic Progression. Sawant S, Dongre H, Singh AK, Joshi S, Costea DE, Mahadik S, Ahire C, Makani V, Dange P, Sharma S, Chaukar D, Vaidya M. PLoS One. 2016 Aug 8;11(8):e0160615. doi: 10.1371/journal.pone.0160615.

Phenotypic Plasticity Determines Cancer Stem Cell Therapeutic Resistance in Oral Squamous Cell Carcinoma.  Biddle A, Gammon L, Liang X, Costea DE, Mackenzie IC., EBioMedicine. 2016 Jan 9;4:138-45. doi: 10.1016/j.ebiom.2016.01.007. eCollection 2016 Feb.

In Vivo Host Response and Degradation of Copolymer Scaffolds Functionalized with Nanodiamonds and Bone Morphogenetic Protein 2. Suliman S, Sun Y, Pedersen TO, Xue Y, Nickel J, Waag T, Finne-Wistrand A, Steinmüller-Nethl D, Krueger A, Costea DE, Mustafa K. , Adv Healthc Mater. 2016 Mar;5(6):730-42. doi: 10.1002/adhm.201500723.

Vimentin-mediated regulation of cell motility through modulation of beta4 integrin protein levels in oral tumor derived cells. Dmello C, Sawant S, Alam H, Gangadaran P, Tiwari R, Dongre H, Rana N, Barve S, Costea DE, Chaukar D, Kane S, Pant H, Vaidya M.Int J Biochem Cell Biol. 2016 Jan;70:161-72. doi: 10.1016/j.biocel.2015.11.015.



Sapkota D, Bruland O, Parajuli H, Osman TA, Teh MT, Johannessen AC, Costea DE.

S100A16 promotes differentiation and contributes to a less aggressive tumor

phenotype in oral squamous cell carcinoma. BMC Cancer. 2015 Sep 9;15:631. doi:

10.1186/s12885-015-1622-1. PubMed PMID: 26353754; PubMed Central PMCID:



Osman TA, Parajuli H, Sapkota D, Ahmed IA, Johannessen ACh, Costea DE. The

low-affinity nerve growth factor receptor p75NTR identifies a transient stem

cell-like state in oral squamous cell carcinoma cells. J Oral Pathol Med. 2015

Jul;44(6):410-9. doi: 10.1111/jop.12251. Epub 2014 Sep 12. PubMed PMID: 25212757.


Min A, Zhu C, Peng S, Rajthala S, Costea DE, Sapkota D. MicroRNAs as Important

Players and Biomarkers in Oral Carcinogenesis. Biomed Res Int. 2015;2015:186904.

doi: 10.1155/2015/186904. Epub 2015 Oct 4. Review. PubMed PMID: 26504785; PubMed

Central PMCID: PMC4609509.




Liang X, Graham KA, Johannessen AC, Costea DE, Labeed FH. Human oral cancer

cells with increasing tumorigenic abilities exhibit higher effective membrane

capacitance. Integr Biol (Camb). 2014 May;6(5):545-54. doi: 10.1039/c3ib40255j.

PubMed PMID: 24663430.


Liang X, Osman TA, Sapkota D, Neppelberg E, Lybak S, Liavaag PG, Johannessen

AC, Jacobsen HK, Enger PØ, Costea DE, Wang J. Rapid adherence to collagen IV

enriches for tumour initiating cells in oral cancer. Eur J Cancer. 2014

Dec;50(18):3262-70. doi: 10.1016/j.ejca.2014.09.010. Epub 2014 Oct 10. PubMed

PMID: 25310894.


Yang N, Yan T, Zhu H, Liang X, Leiss L, Sakariassen PØ, Skaftnesmo KO, Huang

B, Costea DE, Enger PØ, Li X, Wang J. A co-culture model with brain

tumor-specific bioluminescence demonstrates astrocyte-induced drug resistance in

glioblastoma. J Transl Med. 2014 Oct 4;12:278. doi: 10.1186/s12967-014-0278-y.

PubMed PMID: 25280402; PubMed Central PMCID: PMC4198700.



Costea DE, Hills A, Osman AH, Thurlow J, Kalna G, Huang X, Pena Murillo C,

Parajuli H, Suliman S, Kulasekara KK, Johannessen AC, Partridge M. Identification

of two distinct carcinoma-associated fibroblast subtypes with differential

tumor-promoting abilities in oral squamous cell carcinoma. Cancer Res. 2013 Jul

1;73(13):3888-901. doi: 10.1158/0008-5472.CAN-12-4150. Epub 2013 Apr 18. PubMed

PMID: 23598279.


Kidwai F, Costea DE, Hutchison I, Mackenzie I. The effects of CD44

down-regulation on stem cell properties of head and neck cancer cell lines. J

Oral Pathol Med. 2013 Oct;42(9):682-90. doi: 10.1111/jop.12076. Epub 2013 Apr 30.

PubMed PMID: 23627635.


Salo S, Bitu C, Merkku K, Nyberg P, Bello IO, Vuoristo J, Sutinen M,

Vähänikkilä H, Costea DE, Kauppila JH, Lehenkari P, Dayan D, Vered M, Risteli J,

Salo T. Human bone marrow mesenchymal stem cells induce collagen production and

tongue cancer invasion. PLoS One. 2013 Oct 21;8(10):e77692. doi:

10.1371/journal.pone.0077692. eCollection 2013. Erratum in: PLoS One. 2013;8(11).

doi:10.1371/annotation/2bacf09d-7bb8-43ac-a098-b3b9e486d854. Kauppila, Joonas

[corrected to Kauppila, Joonas H]. PubMed PMID: 24204919; PubMed Central PMCID:



Hwang S, Mahadevan S, Qadir F, Hutchison IL, Costea DE, Neppelberg E, Liavaag

PG, Waseem A, Teh MT. Identification of FOXM1-induced epigenetic markers for head

and neck squamous cell carcinomas. Cancer. 2013 Dec 15;119(24):4249-58. doi:

10.1002/cncr.28354. Epub 2013 Oct 1. PubMed PMID: 24114764.


Sapkota D, Costea DE, Ibrahim SO, Johannessen AC, Bruland O. S100A14

interacts with S100A16 and regulates its expression in human cancer cells. PLoS

One. 2013 Sep 27;8(9):e76058. doi: 10.1371/journal.pone.0076058. eCollection

PubMed PMID: 24086685; PubMed Central PMCID: PMC3785438.


Osman TA, Øijordsbakken G, Costea DE, Johannessen AC. Successful triple

immunoenzymatic method employing primary antibodies from same species and same

immunoglobulin subclass. Eur J Histochem. 2013 Sep 16;57(3):e22. doi:

10.4081/ejh.2013.e22. PubMed PMID: 24085271; PubMed Central PMCID: PMC3794353.



Dabija-Wolter G, Bakken V, Cimpan MR, Johannessen AC, Costea DE. In vitro

reconstruction of human junctional and sulcular epithelium. J Oral Pathol Med.

2013 May;42(5):396-404. doi: 10.1111/jop.12005. Epub 2012 Sep 5. PubMed PMID:

22947066; PubMed Central PMCID: PMC3664418.


Litlekalsoy J, Hostmark JG, Costea DE, Illemann M, Laerum OD. Time-dependent

biological differences in molecular markers of high-grade urothelial cancer over

7 decades (ras proteins, pTEN, uPAR, PAI-1 and MMP-9). Virchows Arch. 2012

Nov;461(5):541-51. doi: 10.1007/s00428-012-1323-y. Epub 2012 Oct 6. PubMed PMID:



Dayan D, Salo T, Salo S, Nyberg P, Nurmenniemi S, Costea DE, Vered M.

Molecular crosstalk between cancer cells and tumor microenvironment components

suggests potential targets for new therapeutic approaches in mobile tongue

cancer. Cancer Med. 2012 Oct;1(2):128-40. doi: 10.1002/cam4.24. Epub 2012 Aug 16.

PubMed PMID: 23342263; PubMed Central PMCID: PMC3544451.


Teh MT, Hutchison IL, Costea DE, Neppelberg E, Liavaag PG, Purdie K, Harwood

C, Wan H, Odell EW, Hackshaw A, Waseem A. Exploiting FOXM1-orchestrated molecular

network for early squamous cell carcinoma diagnosis and prognosis. Int J Cancer.

2013 May 1;132(9):2095-106. doi: 10.1002/ijc.27886. Epub 2012 Oct 25. PubMed

PMID: 23034676.


Osman TA, Costea DE, Johannessen AC. The use of salivary cytokines as a

screening tool for oral squamous cell carcinoma : A review of the literature. J

Oral Maxillofac Pathol. 2012 May;16(2):256-61. doi: 10.4103/0973-029X.99083.

PubMed PMID: 22923900; PubMed Central PMCID: PMC3424944.



Dabija-Wolter G, Sapkota D, Cimpan MR, Neppelberg E, Bakken V, Costea DE.

Limited in-depth invasion of Fusobacterium nucleatum into in vitro reconstructed

human gingiva. Arch Oral Biol. 2012 Apr;57(4):344-51. doi:

10.1016/j.archoralbio.2011.09.015. Epub 2011 Oct 24. PubMed PMID: 22024403.


Biddle A, Liang X, Gammon L, Fazil B, Harper LJ, Emich H, Costea DE,

Mackenzie IC. Cancer stem cells in squamous cell carcinoma switch between two

distinct phenotypes that are preferentially migratory or proliferative. Cancer

Res. 2011 Aug 1;71(15):5317-26. doi: 10.1158/0008-5472.CAN-11-1059. Epub 2011 Jun

  1. PubMed PMID: 21685475.


Sapkota D, Bruland O, Costea DE, Haugen H, Vasstrand EN, Ibrahim SO. S100A14

regulates the invasive potential of oral squamous cell carcinoma derived

cell-lines in vitro by modulating expression of matrix metalloproteinases, MMP1

and MMP9. Eur J Cancer. 2011 Mar;47(4):600-10. doi: 10.1016/j.ejca.2010.10.012.

Epub 2010 Nov 10. PubMed PMID: 21074410.


Wiklund ED, Gao S, Hulf T, Sibbritt T, Nair S, Costea DE, Villadsen SB,

Bakholdt V, Bramsen JB, Sørensen JA, Krogdahl A, Clark SJ, Kjems J. MicroRNA

alterations and associated aberrant DNA methylation patterns across multiple

sample types in oral squamous cell carcinoma. PLoS One. 2011;6(11):e27840. doi:

10.1371/journal.pone.0027840. Epub 2011 Nov 22. PubMed PMID: 22132151; PubMed

Central PMCID: PMC3222641.


Sapkota D, Costea DE, Blø M, Bruland O, Lorens JB, Vasstrand EN, Ibrahim SO.

S100A14 inhibits proliferation of oral carcinoma derived cells through G1-arrest.

Oral Oncol. 2012 Mar;48(3):219-25. doi: 10.1016/j.oraloncology.2011.10.001. Epub

2011 Oct 26. PubMed PMID: 22032898.


Mulhall HJ, Labeed FH, Kazmi B, Costea DE, Hughes MP, Lewis MP. Cancer,

pre-cancer and normal oral cells distinguished by dielectrophoresis. Anal Bioanal

Chem. 2011 Nov;401(8):2455-63. doi: 10.1007/s00216-011-5337-0. Epub 2011 Aug 30.

PubMed PMID: 21877186.


2010 and beyond

Harper LJ, Costea DE, Gammon L, Fazil B, Biddle A, Mackenzie IC. Normal and

malignant epithelial cells with stem-like properties have an extended G2 cell

cycle phase that is associated with apoptotic resistance. BMC Cancer. 2010 Apr

28;10:166. doi: 10.1186/1471-2407-10-166. PubMed PMID: 20426848; PubMed Central

PMCID: PMC2868812.


Berge G, Costea DE, Berg M, Rasmussen H, Grotterød I, Lothe RA, Mælandsmo GM,

Flatmark K. Coexpression and nuclear colocalization of metastasis-promoting

protein S100A4 and p53 without mutual regulation in colorectal carcinoma. Amino

Acids. 2011 Oct;41(4):875-84. doi: 10.1007/s00726-010-0514-6. Epub 2010 Feb 27.

PubMed PMID: 20191297.


Lukandu OM, Neppelberg E, Vintermyr OK, Johannessen AC, Costea DE. Khat

alters the phenotype of in vitro-reconstructed human oral mucosa. J Dent Res.

2010 Mar;89(3):270-5. doi: 10.1177/0022034509354980. Epub 2009 Dec 31. PubMed

PMID: 20044580.


Costea DE, Lukandu O, Bui L, Ibrahim MJ, Lygre R, Neppelberg E, Ibrahim SO,

Vintermyr OK, Johannessen AC. Adverse effects of Sudanese toombak vs. Swedish

snuff on human oral cells. J Oral Pathol Med. 2010 Feb;39(2):128-40. doi:

10.1111/j.1600-0714.2009.00825.x. Epub 2009 Oct 4. PubMed PMID: 19804503.


Kulasekara KK, Lukandu OM, Neppelberg E, Vintermyr OK, Johannessen AC, Costea

  1. Cancer progression is associated with increased expression of basement

membrane proteins in three-dimensional in vitro models of human oral cancer. Arch

Oral Biol. 2009 Oct;54(10):924-31. doi: 10.1016/j.archoralbio.2009.07.004. Epub

2009 Aug 11. PubMed PMID: 19674736.


Lukandu OM, Bredholt T, Neppelberg E, Gjertsen BT, Johannessen AC, Vintermyr

OK, Costea DE. Early loss of mitochondrial inner transmembrane potential in

khat-induced cell death of primary normal human oral cells. Toxicology. 2009 Sep

19;263(2-3):108-16. doi: 10.1016/j.tox.2009.06.024. Epub 2009 Jul 8. PubMed PMID:



Dabija-Wolter G, Cimpan MR, Costea DE, Johannessen AC, Sørnes S, Neppelberg

E, Al-Haroni M, Skaug N, Bakken V. Fusobacterium nucleatum enters normal human

oral fibroblasts in vitro. J Periodontol. 2009 Jul;80(7):1174-83. doi:

10.1902/jop.2009.090051. PubMed PMID: 19563299.


Costea DE, Gammon L, Kitajima K, Harper L, Mackenzie IC. Epithelial stem

cells and malignancy. J Anat. 2008 Jul;213(1):45-51. doi:

10.1111/j.1469-7580.2008.00895.x. Review. PubMed PMID: 18638069; PubMed Central

PMCID: PMC2475555.


Gaballah K, Costea DE, Hills A, Gollin SM, Harrison P, Partridge M. Tissue

engineering of oral dysplasia. J Pathol. 2008 Jul;215(3):280-9. doi:

10.1002/path.2360. PubMed PMID: 18484684.


Lukandu OM, Costea DE, Neppelberg E, Johannessen AC, Vintermyr OK. Khat

(Catha edulis) induces reactive oxygen species and apoptosis in normal human oral

keratinocytes and fibroblasts. Toxicol Sci. 2008 Jun;103(2):311-24. doi:

10.1093/toxsci/kfn044. Epub 2008 Feb 29. PubMed PMID: 18310640.


Lukandu OM, Costea DE, Dimba EA, Neppelberg E, Bredholt T, Gjertsen BT,

Vintermyr OK, Johannessen AC. Khat induces G1-phase arrest and increased

expression of stress-sensitive p53 and p16 proteins in normal human oral

keratinocytes and fibroblasts. Eur J Oral Sci. 2008 Feb;116(1):23-30. doi:

10.1111/j.1600-0722.2007.00508.x. PubMed PMID: 18186728.


Partridge M, Costea DE, Huang X. The changing face of p53 in head and neck

cancer. Int J Oral Maxillofac Surg. 2007 Dec;36(12):1123-38. Epub 2007 Sep 17.

Review. PubMed PMID: 17875383.


Neppelberg E, Costea DE, Vintermyr OK, Johannessen AC. Dual effects of sodium

lauryl sulphate on human oral epithelial structure. Exp Dermatol. 2007

Jul;16(7):574-9. PubMed PMID: 17576237.


Costea DE, Tsinkalovsky O, Vintermyr OK, Johannessen AC, Mackenzie IC. Cancer

stem cells – new and potentially important targets for the therapy of oral

squamous cell carcinoma. Oral Dis. 2006 Sep;12(5):443-54. Review. Erratum in:

Oral Dis. 2006 Nov-Dec;12(6):584. PubMed PMID: 16910914.


Costea DE, Kulasekara K, Neppelberg E, Johannessen AC, Vintermyr OK.

Species-specific fibroblasts required for triggering invasiveness of partially

transformed oral keratinocytes. Am J Pathol. 2006 Jun;168(6):1889-97. PubMed

PMID: 16723704; PubMed Central PMCID: PMC1606610.


Costea DE, Johannessen AC, Vintermyr OK. Fibroblast control on epithelial

differentiation is gradually lost during in vitro tumor progression.

Differentiation. 2005 Apr;73(4):134-41. PubMed PMID: 15901281.


Costea DE, Dimba AO, Loro LL, Vintermyr OK, Johannessen AC. The phenotype of

in vitro reconstituted normal human oral epithelium is essentially determined by

culture medium. J Oral Pathol Med. 2005 Apr;34(4):247-52. PubMed PMID: 15752261.


Dimba EA, Gjertsen BT, Bredholt T, Fossan KO, Costea DE, Francis GW,

Johannessen AC, Vintermyr OK. Khat (Catha edulis)-induced apoptosis is inhibited

by antagonists of caspase-1 and -8 in human leukaemia cells. Br J Cancer. 2004

Nov 1;91(9):1726-34. PubMed PMID: 15477863; PubMed Central PMCID: PMC2409956.


Costea DE, Loro LL, Dimba EA, Vintermyr OK, Johannessen AC. Crucial effects

of fibroblasts and keratinocyte growth factor on morphogenesis of reconstituted

human oral epithelium. J Invest Dermatol. 2003 Dec;121(6):1479-86. PubMed PMID: